ANALISIS KADAR OKSALAT PADA DUA JENIS TANAMAN KALE (Brassica oleracea var. acephala dan Brassica oleracea var. palmifolia ) DENGAN METODE SPEKTROFOTOMETRI UV

  • Emma Emawati Universitas Bhakti Kencana
  • Deden Indradinata Fakultas Farmasi Universitas Bahkti Kencana Bandung Indonesia
  • Dina Yulia Agustina Fakultas Farmasi Universitas Bahkti Kencana Bandung Indonesia
Keywords: kale, oxalate, UV spectrophotometry

Abstract

Vegetables have an important function for metabolic and physiological processes in the body. Kale is dubbed as a super food because it has great health benefits. Kale contains nutrients such as vitamins A, C, potassium, calcium, iron, and manganese. Although vegetables have a lot of nutritional content, they also contain anti-nutrients, one of which is oxalic acid. Oxalic acid can irritate the intestines and can be fatal if taken in high doses, so it should be limited to 0.60-1.25 g per day. The purpose of this study was to determine the levels of oxalate in kale by UV spectrophotometry method measured at a wavelength of 351 nm. The product of the reaction is I3 which is proportional to the oxalate concentration. This research begins with method validation. The results of the validation of the calibration curve analysis method have a regression line equation y = 0.4943x + 0.244 with a correlation coefficient 0.998. The detection limit and quantization limit are 0.4018 and 1.3395. The coefficient of variance is 0.8180 and the percent recovery is 89, 92 and 101%. The results of this study showed that the oxalate content in curly kale was 0.6911 mg/100g and kale nero was 1.7045 mg/100g.

References

Agustin, H., & Ichniarsyah, A. N. (2019). Efektivitas KNO3 Terhadap Pertumbuhan Dan Kandungan Vitamin C Kale. Agrin: Jurnal Penelitian Pertanian, 22(1), 46-55.

Agustin, H., & Fauzi, A. R. (2019). Induksi Pembungaan Kale Dengan Aplikasi Pupuk N, P Dan Pemberian Hormon Giberelin

Chamjangali, M.A, et al, 2006, Kinetic Spectrophotometric Method For The Determination of Trace Amouns of Oxalate by an Activation Effect, Analitycal Scienes, The Japan Society For Analitycal Chemestry

Emawati, E., 2018. Analisis kadar oksalat dari teh segar dan teh olahan terhadap lama penyeduhan menggunakan metode spektrofotometri uv. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi, 18(2)

.Fitriani, H., Nurlailah, N., & Rakhmina, D. (2016). Kandungan Asam Oksalat Sayur Bayam. Medical Laboratory Technology Journal, 2(2), 51-55.

Harmita, H., 2004. Petunjuk Pelaksanaan Validasi Metode Dan Cara Perhitungannya. Maj. Ilmu Kefarmasian 1, 117–135. https://doi.org/10.7454/psr.v1i3.3375

Habibah, N., Dhayanaputri, I G. A. S., Karta, I. W., Dewi, N. N. A., (2018): Analisis Kuantitatif Kadar Nitrit dalam Produk Daging Olahan di Wilayah Denpasar Dengan Metode Griess Secara Spektrofotometri, International Journal of Natural Sciences and Engineering, (2017) P-ISSN: 2615-1383 E-ISSN: 2549-6395, Vol 2, NO.1.

Lingga, L. (2010). Cerdas Memilih Sayuran . Jakarta: PT. AgroMedia Pustaka.

Mawaddah, Aida, Roto Roto, and Adhitasari

Suratman. 2017. “Pengaruh Penambahan Urea Terhadap Peningkatan Pencemaran Nitrit Dan Nitrat Dalam Tanah (Influence of Addition of Urea to Increased Pollution of Nitrite and Nitrate in The Soil).” Jurnal Manusia Dan Lingkungan 23(3):360. doi: 10.22146/jml.22473.

Migliozzi, M., Thavarajah, D., Thavarajah, P., & Smith, P. (2015). Lentil and kale: Complementary nutrient-rich whole food sources to combat micronutrient and calorie malnutrition. Nutrients, 7(11), 9285-9298.

Riyanto, (2014) Valdasi dan Verivikasi Metode uji sesuai dengan ISO/IEC 17025 Laboratorium Pengujian dan Kalibrasi -Ed.1, Cet. 1-- Yogyakarta:

Published
2022-03-29
How to Cite
Emawati, E., Indradinata, D., & Agustina, D. (2022). ANALISIS KADAR OKSALAT PADA DUA JENIS TANAMAN KALE (Brassica oleracea var. acephala dan Brassica oleracea var. palmifolia ) DENGAN METODE SPEKTROFOTOMETRI UV. Jurnal Ilmiah Ibnu Sina, 7(1), 38-45. https://doi.org/10.36387/jiis.v7i1.798
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Artikel