AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL UMBI TAWAS UT (Ampelocissus rubiginosa Lauterb.) PADA MENCIT SECARA TOPIKAL
DOI:
https://doi.org/10.36387/jiis.v4i1.245Keywords:
Tawas Ut Tuber (Ampelocissus rubiginosa Lauterb), anti-inflammatory, carageenanAbstract
Tawas ut tuber (Ampelocissus rubiginosa Lauterb.) contains of flavonoid, tanin, compound and saponin which is potentially anti-inflammatory. This objective of this research is to prove by effect giving extrak ethanol tawas ut tuber about effect anti-inflammatory topical based on consideration the fold of the skin and amount of cell neutrofil to mice which induced by carageenan. Thirty mice ware divided into 6 groups. They were normal control group (palacebo gel), positive control (Voltaren®), negative control (karagenin), and testing groups with gel extract concentrations of 0,5%, 1%, and 2%. Ethanol extract of Tawas ut tuber leaf applied, after back of test animals was induced by 0,2 ml of 3% carageenan, then every hour was measured for middosal skin fold thickness during 6 hours observation. The animal’s skin was cut off to perform test at 24 hours after treatment. The neutrophils migration was observed using hematoxylin and eosin (HE) stining method. The percentage result of inhibition (%PI) ethanol extrak of tawas ut tuber with concentration 0,5%, 1%, and 2% were 20,00%; 23,51%; and 24,92% respetively. The average result of netrofil total from ethanol extrak of tawas ut tuber with concentration 0,5%, 1% and 2% were 28,28; 20,88; and 16,24 respecively. Gel 2% posesses the most effective topical anti-inflammatory. Based on the result, it can be concluded that ethanol extrak of tawas ut tuber posesses anti-inflammatory in skin oedema of back of mice induced by carageenan.
References
Anwar, K., D.F. Widodo, Nurlely, L. Triyasmono, Sudarsono, and A.E. Nugroho. 2018. Aktivitas gel ekstrak etanol umbi akar tawas ut (Ampelocissus rubiginosa L.) terhadap penyembuhan luka insisi pada tikus wistar. Trad. Med. J. 23: 30-39
Cintika, K. D. 2015. Uji Efek Antiinflamasi Ekstrak etanol Daun Trengguli (Cassia fistula L.) Pada Edema Punggung Mencit Terinduksi Karagenin. Skripsi. Fakultas Farmasi, Universitas Sanata Dharma, Yogyakarta.
Corwin, E. J. 2008. Handbook Of Pathophysiology. Third Edition. Columbus.
Das, S., P. K. Haldar & G. Pramanik. 2011. Formulation and Evaluation of Herbal Gel Containing Clerodendron infortunatum Leaves Extract. International Journal of PharmTech Research. 3: 140-143.
De Oliveira, C. A. C, Perez, Merino, Prieto & Alvarez. 2001. Protective Effects of Panax Ginseng on Muscle Injury and Inflammation After Eccentric Exercise. Comparative Biochemistry and Physiology. 130 : 369-377.
Erlina, R., A. Indah & Yanwirasti. 2007. Efek Antiinflamasi Ekstrak Etanol Kunyit (Curcuma domestika Val.) Pada Tikus Jantan Galur Wistar. Journal Sains dan Teknologi Farmasi. 12 : 112-115.
Fitriyani, A., L. Winarti., S. Muslichah & Nuri. 2011. Uji Antiinflamasi Ekstrak Metanol Daun Sirih Merah (Piper crocatum Ruiz & Pav) Pada Tikus Putih. Fakultas Farmasi, Universitas Jember. Majalah Obat Tradisional. 16 : 34-42.
Ganiswarna, S. G. 2008. Farmakologi dan Terapi. Edisi V Editor Sulista Gan Gunawan. Balai Penerbit FKUI. Jakarta.
Harvey, R. A., P. C. Champe. 2006. Lippincott’s Illustrated. Reviews : Pharmacology. Lippincott Williams & Wilkins. Philadelphia.
Hidayati, N. A., S. Listyawati & A. D. Setyawan. 2008. Kandungan Kimia dan Uji Antiinflamasi Ekstrak Etanol Lantana Camara L. Pada Tikus Putih (Rattus Norvegicus L.) Jantan. Bioteknologi. 5 : 10-17.
Katzung, B. G., S. B. Masters. & A. J. Trevor. 2009. Basic and Clinical Pharmacology. Edisi 11. New York : Mc Graw-Hill.
Krismawati, A & M. Sabran. 2006. Pengelolaan Sumber Daya Genetik Tanaman Obat Spesifik Kalimantan Tengah. Buletin Plasma Nutfah. 12 : 16-23.
Latifah, A. 2010. Kajian Farmakognostik Akar Tawas Ut (Ampelocissus rubiginosa L.) Asal Kalimantan Tengah. Skripsi Program Studi Farmasi, Universitas Lambung Mangkurat, Banjarbaru.
Majumdar, S., R. Dave. 2013. Formulation Study of Gel Containing Pterarpus Santalinus Extrat For Its Antiinflammatory Activity. World Journal of Pharmacy and Pharmaceutical Sciences. Vol 2 (6) : 4951-4964
Nagori, B. D. & R. Solanki. 2011. Role of Medicina Plants in Wound Healing. Research Journal of Medicinal Plant. 5 : 392-450
Nanda, Y., M. N. Salim., C. D. Iskandar. 2017. Histopatologi Kulit Mencit (Mus musculus) Fase Remodeling Pada Penyembuhan Luka Sayat dengan Salep Getah Jarak Pagar (Jatropha curcas Linn). JIMVET. 01 (4) : 780-787.
Narayana, K. R, Reddy, & Chaluvadi. 2001. Bioflavonoids Classification, Pharmacological, Biochemical Effects and Therapeutic Potential. Indian Journal Pharmacology. 2-16
Novieanty, K. 2017. Penetapan Kadar Fenol dan Flavonoid Total Serta Aktivitas Antioksidan Ekstrak Etanol Akar Tawas Ut (Ampelocissus rubiginosa L.). Skripsi Program Studi Farmasi, Universitas Lambung Mangkurat, Banjarbaru.
Olabissi, O. A, O. Moussa, O. Moustapha, Z. F. Edgard, K. Eleonore, L. Marius & G. I. Pierre. 2011. Acute Toxicity and Anti-inflammatory Activity of Aqueous Ethanol Extract of Root Bark of Ximenia Americana L. (Olacaceae). African Journal of Pharmacy and Pharmacology. 5 (7) : 807-811.
Petel, M., Murugananthan & S. K. P. Gowda. 2012. In Vivo Animal Models In Preclinical Evaluation of Anti-Inflammatory Activity- A Review. A Review. International Journal of Pharmaceutical Research & Allied Sciences. 1 : 01-05.
Pratiwi, D., S. Wahdaningsih & Isnindar. 2013. Uji Aktivitas Antioksidan Daun Bawang Mekah (Eleutherine amaricana Merr.) dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil). Traditional Medicine Journal. 18 (1) : 9-16
Sostales, D. 2016. Uji Efek Antiinflamasi Topikal Ekstrak Etanol Daun Majapait (Crescetia cujete L.) Terhadap Jumlah Neutrofil dan Ekspresi Siklooksigenase 2 Pada Mencit Terinduksi Karagenin. Skripsi. Fakultas Farmasi, Universitas Sanata Dharma. Yogyakarta.
Suirta, I., W., N. M. Puspawati & I. A. R. A. Asih. 2016. Aktifitas Antiinflamasi Topikal Minyak Atsirih dan Ekstrak Eter Tumbuhan Tenggulun (Protium Javanicum Burm.) Terhadap Model Inflamasi Kulit Pada Tikus. Journal Of Applled Chemistry. 4 : 2302-7274.
Syamsuni, A. 2006. Ilmu Resep. Penerbit Buku Kedokteran EGC. Jakarta.
Tiwari, P., B. Kumar., M. Kaur., G. Kaur., H. Kaur. 2011. Phytochemical Screening and Extraction : A Review. International Pharmaceutica Sciencia. 1: 98-106.
Utami, S., A. 2016. Uji Efek Antiinflamasi Topikal Ekstrak Milk Thistle Pada Jumlah Neutrofil dan Ekspresi COX-2 Mencit Betina Terinduksi Karegenin. Skripsi. Fakultas Farmasi, Universitas Sanata Dharma, Yogyakarta.
Zerlina, F. D. 2013. Uji Aktivitas Ekstrak Akar Tawas Ut (Ampelocissus rubiginosa L.) Sebagai Hepatoprotektor terhadap Mencit Putih Jantan Balb/C yang diinduksi Karbon tetraklorida (CCL4). Skripsi. Program Studi Farmasi, Universitas Lambung Mangkurat, Banjarbaru.
