EFEKTIVITAS KOMBINASI PLASMA JET NON -THERMAL DAN SPRAY Aloe vera (L). Burm. f . PADA PENYEMBUHAN LUKA DIABETES
DOI:
https://doi.org/10.36387/jiis.v6i2.708Keywords:
A. vera 1% spray, plasma jet non thermal, diabetic wounds, malondialdehid, the skin wound ratio, blood profileAbstract
Plasma jet non thermal has benefit in wound healing, but less control can to accumulation of reactive oxygen species. This study aims to determine the effectiveness of the combination of plasma jet and A. vera spray 1% for wound healing in diabetic wounds mice model with parameters of malondialdehyde levels, the skin wound ratio, and blood profile. This study used male Balb/C were induced by a streptozotocin (STZ) and divided into 4 groups, namely groups of untreated diabetic mice wounds (N), groups of diabetic mice wound with A. vera spray 1% (Av), groups of diabetic mice wound with plasma jet non thermal at distance of 20 mm for 3 minutes (P), and groups of diabetic mice wound with plasma jet non thermal at distance of 20 mm for 3 minutes and A. vera spray 1% (PAv). The solution of A. vera spray 1% preparation consists of A. vera, DMSO, and aquabidest extracts. The wounds of all groups treated for 14 days then the malondialdehyde, skin wound ratio and blood profile were calculated by SPSS Statistic. The results of blood glucose before treatment were 241.74 dL ± 51.99. The combination of plasma jet and A. vera spray 1% groups were significant (p <0.05) in reduce malondialdehyde levels, reduced the the skin wound ratio but not different significantly (p> 0.05), and not effect on blood profile data (p> 0.05). In conclusion, A. vera spray 1% can reduce the side effects of plasma jet in diabetic wounds by reducing malondialdehyde levels.
References
Damayanti, R. A., & Yuwono, T. (2014). Dimetilsulfoksid Sebagai Enhancer Transpor Transdermal Teofilin Sediaan Gel. Pharmaciana, 3(1), 66–68.
Darmawati, S., Rohmani, A., Nurani, L. H., Prastiyanto, M. E., Dewi, S. S., Salsabila, N., … Nasruddin, N. (2019). When plasma jet is effective for chronic wound bacteria inactivation, is it also effective for wound healing? Clinical Plasma Medicine, 14(18), 100085. https://doi.org/10.1016/j.cpme.2019.100085
Deeds, M. C., Anderson, J. M., Armstrong, A. S., Gastineau, D. A., Hiddinga, H. J., Jahangir, A., … Kudva, Y. C. (2011). Single dose streptozotocin-induced diabetes: Considerations for study design in islet transplantation models. Laboratory Animals, 45(3), 131–140. https://doi.org/10.1258/la.2010.010090
Haritha Yadav, K. C., Ravi Kumar, J., Basha, I. I., Deshmukh, G. R., Gujjula, R., & Santhamma, B. (2012). Wound healing activity of topical application of Aloe vera gel in experimental animal models. International Journal of Pharma and Bio Sciences, 3(2), 66–67.
Hekmatpou, D., Mehrabi, F., Rahzani, K., & Aminiyan, A. (2019). The effect of aloe vera clinical trials on prevention and healing of skin wound: A systematic review. Iranian Journal of Medical Sciences, 44(1), 3–7.
Hussein, M. U., & Yousif, E. S. (2018). Mechanism of Platelets Stimulation by Non Thermal Plasma. International Journal of Chinese Medicine, 2(February), 1–5. https://doi.org/10.11648/j.ijcm.20180201.11
Keohane, E. M., Smith, L., & Walenga, J. M. (2016). Rodak’s Hematology: Clinical Principles and ApplicationsNo Title (Edisi ke-5). Elsevier 232-234.
Nasruddin, Nakajima, Y., Mukai, K., Setyowati, H., Rahayu, E., Sugama, J., & Nakatani, T. (2014). Cold plasma on full-thickness cutaneous wound accelerates healing through promoting in fl ammation , re-epithelialization and wound contraction. Clinical Plasma Medicine, 2(1), 28–35. https://doi.org/10.1016/j.cpme.2014.01.001
Nku, C. O., Ofem, O. E., & Okon, U. A. (2014). Effect of prolong consumption of crude Aloe barbadensis (Aloe vera) gel on haematological indices in rats. Asian Journal of Medical Sciences, 6(2), 58–60. https://doi.org/10.3126/ajms.v6i2.11106
Nurani, L. H., Nasruddin, N., Darmawati, S., & Wahyuningtyas, E. S. (2020). Plasma Jet – Treated Lidah Buaya (Aloe Vera) Influences Proliferative-Phase Wound Healing. Plasma Medicine, 10(2), 123–138.
Perka BPOM. (2019). Persyaratan Keamanan dan Mutu Obat Tradisional. Badan Pengawas Obat dan Makanan Republik Indonesia Hal 22-23, 16-17.
Prakoso, Y. A. (2018). The Effects of Aloe vera Cream on the Expression of CD4 + and CD8 + Lymphocytes in Skin Wound Healing, 2018.
Sikumbang, I. M., Astuti, R. A., Wahyuningtyas, E. S., Lutfiyati, H., Wijayatri, R., & Nasruddin, N. (2020). Wound healing activity of aloe vera extract spray on acute wound in male balb/c mice. Pharmaciana, 10(3), 315. https://doi.org/10.12928/pharmaciana.v10i3.16640
Subandrate. (2016). Hubungan Kadar Glukosa Darah dengan Peroksidasi Lipid pada Pasien Diabetes Melitus tipe 2. Departemen Biokimia Fakultas Kedokteran Universitas Sriwijaya, 43(7), 487–489.
Von Woedtke, T., Schmidt, A., Bekeschus, S., Wende, K., & Weltmann, K. D. (2019). Plasma medicine: A field of applied redox biology. In Vivo, 33(4), 1011–1026. https://doi.org/10.21873/invivo.11570
Wahyuningtyas, E. S., Setyowati, H., Rahayu, E., & Lutfiyati, H. (2020). Efek Perlakuan Kombinatif Plasma Medis dan Ekstrak Daun Binahong Terhadap Penyembuhan Luka Fase Proliferasi Pada Mencit Diabetik. University Research Colloqium, 238, 239–240.
Widyaningsih, W., Sativa, R., & Primardiana, I. (2015). Efek Antioksidan Ekstrak Etanol Ganggang Hijau (Ulva Lactuca L.) Terhadap Kadar Malondialdehid (MDA) Dan Aktivitas Enzim Superoksida Dismutase (SOD) Hepar Tikus Yang Diinduksi CCL4. Media Farmasi: Jurnal Ilmu Farmasi, 12(2), 168–169. https://doi.org/10.12928/mf.v12i2.3756
Xu, D., Wang, S., Li, B., Qi, M., Feng, R., Li, Q., & Zhang, H. (2020). E ff ects of Plasma-Activated Water on Skin Wound Healing in Mice. Microorganisms, 8, 2–14.
