AKTIVITAS SITOTOKSIK EKSTRAK ETIL ASETAT DAUN AKAR KAIK-KAIK (Uncaria cordata (Lour.) Merr) PADA SEL KANKER SERVIKS HeLa
DOI:
https://doi.org/10.36387/bv4qqc35Keywords:
Cytotoxic, Ethyl acetate extract, HeLa cells, MTT assay, Uncaria cordataAbstract
Cervical cancer is one of the leading causes of death in women worldwide and is a highly prevalent disease in developing countries. Efforts to prevent and treat cervical cancer continue to be developed, one of which is through the use of natural ingredients that have anticancer activity. This study aims to determine the cytotoxic activity of ethyl acetate extracts of Kaik-Kaik root leaves (Uncaria cordata (Lour.) Merr) against HeLa cervical cancer cells in vitro. Kaik-Kaik root leaves were extracted using a stepwise maceration method with n-hexane, ethyl acetate, and methanol solvents. The ethyl acetate extract was then tested for its cytotoxic activity using the MTT assay at various concentrations. Phytochemical screening results showed the presence of secondary metabolites such as flavonoids, tannins, saponins, and terpenoids that play a role in biological activity. Cytotoxicity test results showed that the ethyl acetate extract had very strong potential with an IC₅₀ value of 0.524 μg/mL, which is classified as highly toxic to cancer cells. These findings indicate that Uncaria cordata has the potential to be developed as a herbal-based anticancer agent. This study is expected to contribute to the development of safer, more effective, and more affordable alternative therapies for cervical cancer patients.
References
1. Herniyatun, Lestari, Kuntoadi GB, Karlina N, Dewi SU. 2024. Faktor-faktor yang berhubungan dengan kejadian kanker serviks. Ensiklopedia of Journal;6.
2.Anonim. 2025. Cancer. World Health Organization.
3.Anonim. 2024. Cervical cancer. World Health Organization.
4.Deswita S, Rahma EN, Njurumana VC, Yanuarti R. 2022. Pengujian flavonoid total dan uji aktivitas antioksidan fraksi aktif daun akar kaik-kaik (Uncaria cordata (Lour.) Merr) yang berpotensi sebagai obat diare. Jurnal Biologi dan Pembelajarannya (JB&P); 9(2):105–112.
5.Rahmawati N, Utami R, Azwendah. 2016. Isolasi dan uji aktivitas sitotoksik senyawa murni dari ekstrak etil asetat daun tumbuhan akar kaik-kaik (Uncaria cordata (Lour.) Merr). Scientia: Jurnal Farmasi dan Kesehatan;6(2):122.
6.Rahmawati N, Yodha AWM, Karmilah, Nisaa NRK, Fitriana WD, Bahar H, et al. 2024. Konsep dan teori fitokimia. Wahyuni TP, editor. Indonesia: GET Press Indonesia; ISBN: 978-623-198-998-7.
Rhomah AC, Wulandari DN, Bintari YR. 2025. Potensi antioksidan fraksi etil asetat daun ubi jalar ungu (Ipomea batatas L.) dengan menggunakan metode DPPH. J Insan Farm Indonesia;8(2):215-224. doi:10.36387/jifi.v8i2.2513.
8.Lestari P, Irfan N, Indrayoni P, Pristiyantoro, Rahmawati N, Herli MA, et al. 2025. Teknologi farmasi bahan alam. Sepriano, editor. Sonpedia; ISBN: 978-634-265-152-0.
9.Irawan A, Ulfah M, Mansor K.2024. Uji aktivitas antibakteri fraksi etil asetat daun jambu mete (Anacardium occidentale L.) terhadap bakteri Bacillus subtilis dan Pseudomonas aeruginosa. J Insan Farm Indonesia ;7(3):195-205. doi:10.36387/jifi.v7i3.2183.
10.Randan EJ, Rija’i HR, Ahmad I.2023. Skrining fitokimia dan profil KLT antioksidan ekstrak metanol dan ekstrak partisi n-heksana akar bajakah (Uncaria nervosa Elmer). Proceeding of Mulawarman Pharmaceuticals Conferences;1–6.
11.Muslihin N, Usman WF, Rahmawati N, Salam DA, Eriadi A, Rahmawati RP, et al. 2026. Farmakognosi–fitokimia. SastroAtmodjo S, editor. Indonesia: GET Press Indonesia; ISBN: 978-634-255-469-2.
12.CCRC. 2009a. Prosedur Tetap: Cell Thawing. Cancer Chemoprevention Research Center Fakultas Farmasi UGM Yogyakarta :1–3.
13.CCRC. 2009b. Prosedur Tetap: Panen Sel. Cancer Chemoprevention Research Center Fakultas Farmasi UGM Yogyakarta:1–3.
14.CCRC. 2009c.Prosedur Tetap: Perhitungan Sel. Cancer Chemoprevention Research Center Fakultas Farmasi UGM Yogyakarta.
15.CCRC. 2014. Protokol: Uji Sitotoksik Metode MTT. Cancer
Chemoprevention Research Center Fakultas Farmasi UGM Yogyakarta :1–8.
16.CCRC. 2009d. Prosedur Tetap: Preparasi Sampel. Cancer Chemoprevention Research Center Fakultas Farmasi UGM Yogyakarta :1–3.
17.Rahmawati N, Ismail NH, Hamidi D, Wahyuni FS. 2023.Cytotoxic activity screening of various Uncaria spp plants on T47D breast cancer cells. Trop J Nat Prod Res;7(1):2218-2221.
18.Silvia SD, Rahma EN, Njurumana VC, Yanuarti R.2020. Pengujian flavonoid total dan uji aktivitas antioksidan fraksi aktif daun akar kaik-kaik (Uncaria cordata (Lour.) Merr) yang berpotensi sebagai obat diare. Jurnal Biologi dan Pembelajarannya.
19.Islamiyati R, Mugitasari DE, Nafiah LN, Jayanto I. 2024. Uji aktivitas antioksidan ekstrak etil asetat daun matoa menggunakan radikal bebas DPPH (difenilpikrilhidrazil). Pharmacon; 13:611–618.
20.Rahmawati N, Hari DG, Nurbaiti, Rahmawati A, Nursyafni, Indriani L, et al. 2023. Uji aktivitas antiinflamasi ekstrak etil asetat daun tumbuhan akar kaik-kaik (Uncaria cordata (Lour.) Merr) pada tikus putih (Rattus norvegicus) jantan. As-Shiha: Jurnal Kesehatan ;3(1):16-22.
21.Wang X, Wang H, Mou X, Xu Y, Han W, Huang A, Li Y, Jiang H, Yang X, Hu Z. Lysophosphatidic acid protects cervical cancer HeLa cells from apoptosis induced by doxorubicin hydrochloride. Oncology Letters. 2022;24(2):1–8.
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