EVALUASI AKTIVITAS ANTIBAKTERI SABUN VCO pH NETRAL TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli
DOI:
https://doi.org/10.36387/jifi.v8i2.2827Keywords:
Antibacterial activity, Escherichia coli, Liquid soap, Staphyloccus aureus, virgin coconut oilAbstract
Liquid bath soap is widely used as an antiseptic in daily activities, but its generally high pH can erode the skin’s protective barrier and reduce comfort. Saponification is the reaction between a strong base and fatty acids. Virgin Coconut Oil (VCO), which contains lauric acid, is known for its antibacterial properties. This study aimed to determine the characteristics of VCO-based liquid soap and compare its antibacterial effectiveness against two structurally different bacteria, Staphylococcus aureus and Escherichia coli. The research employed a post-test only experimental design with descriptive-quantitative data collection. The stages included organoleptic evaluation, pH measurement, viscosity, specific gravity, and foam height testing. Only samples that fulfilled standard soap characteristics were further tested using the Kirby-Bauer diffusion method. Results showed that the soap met physical requirements, exhibiting a liquid texture, gray color, vanilla aroma, pH 7.08, viscosity of 1370 mPas, specific gravity of 1.01 g/mL, and foam height of 2 cm. The neutral-pH VCO soap demonstrated antibacterial activity against both S. aureus and E. coli. Statistical analysis using the Mann-Whitney test yielded an Asymp.Sig value of 0.083, indicating no significant difference between the antibacterial effects, suggesting broad antibacterial coverage. These findings support the potential of VCO soap for daily hygiene.
References
1. Dalibor. Mijaljica, Spada. Fabrizio HIP. Skin Cleansing without or with Compromise : Molecules. 2022;27.
2. Blaak J, Staib P. The Relation of pH and Skin Cleansing. Curr Probl Dermatology. 2018;54:132–42.
3. Wijaya MA, Cahyanti ND. Pengaruh pH Sabun Cair Virgin Coconut Oil (VCO) Terhadap Aktivitas Antibakteri Staphylococcus aureus. Lontara. 2025;6(1):52–61.
4. Yang HT, Chen JW, Rathod J, Jiang YZ, Tsai PJ, Hung YP, et al. Lauric acid is an inhibitor of Clostridium difficile growth in vitro and reduces inflammation in a mouse infection model. Front Microbiol. 2018;8(JAN):1–16.
5. Casillas-Vargas G, Ocasio-Malavé C, Medina S, Morales-Guzmán C, Del Valle RG, Carballeira NM, et al. Antibacterial fatty acids: An update of possible mechanisms of action and implications in the development of the next-generation of antibacterial agents. Prog Lipid Res. 2021;82(February).
6. Touaitia R, Mairi A, Ibrahim NA, Basher NS, Idres T, Touati A. Staphylococcus aureus: A Review of the Pathogenesis and Virulence Mechanisms. Antibiotics. 2025;14(5):1–37.
7. Pokharel P, Dhakal S, Dozois CM. The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen. Microorganisms. 2023;11(2).
8. Dianti S, Kusumayanti H. Formulation of Antibacterial Liquid Soap Based on Virgin Coconut Oil with Various Concentrations of Carica Concentrate and Potassium Hydroxide Volume. J Vocat Stud Appl Res. 2022;4(1):13–7.
9. Rishliani YR. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Nanas (Ananas comosus (L.,) Merr.) Terhadap Propionibacterium acnes. Jambi; 2022.
10. Badan Standar Nasional Indonesia. Sabun Mandi Cair [Internet]. 06–4058th ed. 2017. Available from: https://akses-sni.bsn.go.id/viewsni/baca/7188.
11. Parente E, Ares G. How do appearance and fragrance influence expectations and conceptual associations of cosmetic products? An exploratory case study with liquid hand soap. J Sens Stud. 2021;36(2).
12. Dianursanti, Fransisca M, Alifia KCH. The effect of adding citric acid on making antibacterial film-formed soap for hospital. AIP Conf Proc. 2020;2255(September).
13. Nurhajawarsih. Formulation and Analysis of Solid Bath Soap With the Addition of Seaweed. J Sains dan Tek Terap [Internet]. 2023;1(1):27–40. Available from: https://journal.akom-bantaeng.ac.id/index.php/jstt
14. Yulianto MFSP, Suprapto S. Modifikasi Polimer Hidroksipropil Metil Selulosa Dan Sodium Tripolifosfat Dengan Metode Taut Silang. Usadha J Pharm. 2025;4(1):91–101.
15. Wirasti W, Ulfah F, Slamet S. Karakterisasi Sediaan Suspensi Nanopratikel Ekstrak Etanol Daun Afrika (Vernonia Amygdalina Del.). Cendekia J Pharm. 2020;4(2):138–48.
16. Klimaszewska E, Wieczorek D, Lewicki S, Stelmasiak M, Ogorzałek M, Szymański Ł, et al. Effect of New Surfactants on Biological Properties of Liquid Soaps. Molecules. 2022;27(17).
17. Hussain MS, Al-Alaq FT, Al-Khafaji NS, Al-Dahmoshi HOM. Antibacterial effect of virgin and refined coconut oils on pathogenic bacteria: A review. Indian J Forensic Med Toxicol. 2020;14(4):6042–8.
18. Bender M•, Buckley •, Sattley •, Stahl •. Brock Biology of Microorganisms FIFTEENTH EDITION. 2019.
19. Niken N, Yusuf RN, Rahayu Y, Ibrahim I. Uji Aktivitas Antibakteri Virgin Coconut Oil (VCO) terhadap Pertumbuhan Bakteri Staphylococcus aureus. Biosci J Ilm Biol. 2023;11(1):405.
20. Snyder L, Champness W. Molecular Genetics of Bacteria. 4th editio. ASM Press; 2013.
21. Islamy AAF, Hendrawati N. Pengaruh Konsentrasi Natrium Hidroksida (Naoh) Dalam Proses Pembuatan Sweet Potato Soap. DISTILAT J Teknol Separasi. 2023;8(4):749–57.
22. Tobe S, Majima T, Tadenuma H, Suekuni T, Sakai K, Sakai H, et al. Nonionic Surfactants Enhancing Bactericidal Activity at Their Critical Micelle Concentrations. J Oleo Sci. 2015;68(1):61–8.
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