METABOLIT ANTIBAKTERI DARI Aspergilalus nidulans DSP 20, JAMUR ENDOFIT MANGROVE ASOSIASI Sesuvium portulacastrum
DOI:
https://doi.org/10.36387/safz8j78Keywords:
antibacterial activity, Aspergillus nidulans, endophytic fungi, mangrove association, secondary metabolite, Sesuvium portulacastrumAbstract
Mangrove-associated endophytic fungi are promising producers of antibacterial secondary metabolites. This study evaluated the potential of Aspergillus nidulans DSP 20, isolated from leaves of Sesuvium portulacastrum collected from the Sekotong mangrove-associated ecosystem, as a producer of antibacterial metabolites. The isolate was obtained through surface sterilization of leaf tissue, colony purification, and morphological characterization. Metabolite production was performed by 21-day solid-state fermentation followed by ethyl acetate extraction. The metabolite profile was qualitatively analyzed using thin-layer chromatography, while antibacterial activity was tested against Staphylococcus aureus and Escherichia coli using the disc diffusion method. DSP 20 showed morphological characteristics supporting its identification as A. nidulans, produced an ethyl acetate extract with several chromatographic spots, and formed inhibition zones against both test bacteria. These findings indicate that A. nidulans DSP 20 is a potential preliminary source of antibacterial metabolites from mangrove-associated endophytic fungi.
References
1. Tarin-Pello A, Suay-Garcia B, Perez-Gracia MT. Antibiotic resistant bacteria: current situation and treatment options to accelerate the development of a new antimicrobial arsenal. Expert Rev Anti Infect Ther. 2022;20:1095-108.
2. Srinivasan R, Kannappan A, Shi C, Lin X. Marine bacterial secondary metabolites: a treasure house for structurally unique and effective antimicrobial compounds. Mar Drugs. 2021;19:530.
3. Jha P, Kaur T, Chhabra I, Panja A, Paul S, Kumar V, et al. Endophytic fungi: hidden treasure chest of antimicrobial metabolites interrelationship of endophytes and metabolites. Front Microbiol. 2023;14:1227830.
4. Palit K, Rath S, Chatterjee S, Das S. Microbial diversity and ecological interactions of microorganisms in the mangrove ecosystem: threats, vulnerability, and adaptations. Environ Sci Pollut Res Int. 2022;29:32467-512.
5. Chen S, Cai R, Liu Z, Cui H, She Z. Secondary metabolites from mangrove-associated fungi: source, chemistry and bioactivities. Nat Prod Rep. 2022;39:560-95.
6. Djamain NS, Anggia V, Fachrunisa N. Uji aktivitas antibakteri gel ekstrak etanol daun angsana (Pterocarpus indicus Willd.) terhadap bakteri Staphylococcus aureus. Jurnal Insan Farmasi Indonesia. 2020;3(1):124-31.
7. Kumalasari E, Agustina D, Ariani N. Uji aktivitas antibakteri ekstrak daun bawang dayak (Eleutherine palmifolia Merr.) terhadap Escherichia coli. Jurnal Insan Farmasi Indonesia. 2020;3(1):75-84.
8. Monica C, Zamzani I, Nashihah S. Aktivitas antibakteri ekstrak etanol 96% daun bangkal (Nauclea subdita) terhadap bakteri Staphylococcus aureus. Jurnal Insan Farmasi Indonesia. 2024;7(2):33-43.
9. Ayuchecaria N, Oksal E, Martani NS, Komara NK, Chuchita, Pereiz Z. Skrining fitokimia dan uji antibakteri ekstrak etanol daun hanjuang merah (Cordyline fruticose) terhadap bakteri Staphylococcus epidermidis. Jurnal Insan Farmasi Indonesia. 2024;7(1):86-94.
10. Brakhage AA, Schroeckh V. Fungal secondary metabolites - strategies to activate silent gene clusters. Fungal Genet Biol. 2011;48:15-22.
11. Inglis DO, Binkley J, Skrzypek MS, Arnaud MB, Cerqueira GC, Shah P, et al. Comprehensive annotation of secondary metabolite biosynthetic genes and gene clusters of Aspergillus nidulans, A. fumigatus, A. niger and A. oryzae. BMC Microbiol. 2013;13:91.
12. Ghazawi KF, Fatani SA, Mohamed SG, Mohamed GA, Ibrahim SRM. Aspergillus nidulans - natural metabolites powerhouse: structures, biosynthesis, bioactivities, and biotechnological potential. Fermentation. 2023;9:325.
13. Safwan S, Hsiao G, Lee TH, Lee CK. Bioactive compounds from an endophytic fungi Nigrospora aurantiaca. Bot Stud. 2021;62:18.
14. Safwan S, Wang SW, Hsiao G, Hsiao SW, Hsu SJ, Lee TH, et al. New trichothecenes isolated from the marine algicolous fungus Trichoderma brevicompactum. Mar Drugs. 2022;20:80.
15. McIntyre M, Dynesen J, Nielsen J. Morphological characterization of Aspergillus nidulans: growth, septation and fragmentation. Microbiology. 2001;147:239-46.
16. Han KH, Lee DB, Kim JH, Kim MS, Han KY, Kim WS, et al. Environmental factors affecting development of Aspergillus nidulans. J Microbiol. 2003;41:34-40.
17. Klejnstrup ML, Frandsen RJN, Holm DK, Nielsen MT, Mortensen UH, Larsen TO, et al. Genetics of polyketide metabolism in Aspergillus nidulans. Metabolites. 2012;2:100-33.
18. Jia SL, Chi Z, Liu GL, Hu Z, Chi ZM. Fungi in mangrove ecosystems and their potential applications. Crit Rev Biotechnol. 2020;40:852-64.
19. Giles SS, Soukup AA, Lauer C, Shaaban M, Lin A, Oakley BR, et al. Cryptic Aspergillus nidulans antimicrobials. Appl Environ Microbiol. 2011;77:3669-75.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Jurnal Insan Farmasi Indonesia
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
