ANTIHIPERURESEMIA FLAVONOID DARI EKSTRAK ETANOL PELEPAH DAUN NIPAH (Nypa fruticans.Wurmb)
DOI:
https://doi.org/10.36387/jiis.v8i3.1681Keywords:
Nypa fruticans Wurmb, Antihyperuresemia, (-)Epicatechin, KaempferolAbstract
Hyperuresimia or gout in advanced stages causes joint damage (gout), kidney stones and kidney damage. Flavonoids as antioxidants can inhibit the activity of the enzyme xanthine oxidase which causes uric acid not to form. Nypa fruticans or known as nipah is traditionally used in the treatment of asthma, leprosy, tuberculosis, sore throat, liver disease, snake bites, as a pain reliever, as a sedative and carminative. The content of flavonoid compounds in nipah plants includes chlorogenic acid, protocatechuic acid, kaempferol, gallic acid, hydroxybenzoic acid, rutin, quercetin, cinnamic acid, and anthocyanins. The purpose of this study was to isolate flavonoid compounds that have potential as antihyperuresemia from nipah fronds. The research process began with the extraction of nipah leaves using 96% ethanol by maceration, fractionation using liquid-liquid partition method, isolation using centrifugal chromatography (chromatotron) method and structure elucidation using 1HNMR, 13CNMR, FTIR and GC-MS. Antihyperuresemia testing was carried out in-vitro by inhibiting xanthine oxidase enzyme activity. From the results of the study, two flavonoid compounds were successfully isolated including (-) epicatechin, and chamferol. The results of measuring the inhibitory activity of xanthine oxidase enzyme obtained IC50 values of (-) epicatechin and chamferol of 3.543 ppm and 10.383 ppm, respectively.
References
Adwas, Almohktar A., Elsayed, A., Azab, A. E., & Quwaydir, F. A. (2019). Oxidative stress and antioxidant mechanisms in human body. J. Appl. Biotechnol. Bioeng, 6(1), 43–47.
Agrawal, Varsha, & Desai, Sonal. (2015). Centrifugally accelerated thin layer chromatography for isolation of marker compounds and bioactives. Journal of Pharmacognosy and Phytochemistry, 3(6), 145–149.
Aisyah, Lilis Siti, Yun, Yenny Febriani, Herlina, Tati, Julaeha, Euis, Zainuddin, Achmad, Nurfarida, Ida, Hidayat, Ace Tatang, Supratman, Unang, & Shiono, Yoshihito. (2017). Flavonoid compounds from the leaves of Kalanchoe prolifera and their cytotoxic activity against P-388 murine leukimia cells. Natural Product Sciences, 23(2), 139–145.
Astuti, Maria Dewi, Nisa, Khairatun, & Mustikasari, Kamilia. (2020). Identification of chemical compounds from nipah (Nypa fruticans Wurmb.) endosperm. BIO Web of Conferences, 20, 3002. EDP Sciences.
Borysova, Inna, & Potapova, Tetyana. (2022). Assessment of functional activity reduction–as a criterion for determining disability in patients with rheumatoid arthritis. Wiadomości Lekarskie, 75(9 p. 1), 2081–2085.
Fachriyah, E., Ghifari, M. A., & Anam, K. (2018). Isolation, Identification, and Xanthine oxidase inhibition activity of alkaloid compound from Peperomia pellucida. IOP Conference Series: Materials Science and Engineering, 349, 12017. IOP Publishing.
Handayani, Marybet Tri Retno, Mumpuni, Esti, & Laksmitawati, Dian Ratih. (2022). Aktivitas Penghambatan Xantin Oksidase Herba Tapak Liman, Biji Jintan Hitam, Dan Daun Talok Secara In Silico Dan In Vitro. Journal of Syntax Literate, 7(5).
Irianti, Tanti Tatang, & Nuranto, Sindu. (2021). Antioksidan dan kesehatan. Ugm Press.
Juliana, Fitriani. (2022). Pengaruh Pemberian Sirup Ekstrak Daun Nipah (Nypa Fruticans Wurmb) Pada Tikus Putih Jantan Sebagai Penurun Kadar Asam Urat. Skripsi. Universitas Al-Irsyad Cilacap.
Katja, Dewa G., Mantiri, Sisilia A., Runtuwene, Max R. J., Supratman, Unang, & Hilmayanti, Erina. (2021). Senyawa Katekin (Flavonoid) dari Kulit Batang Chisocheton balancae C. DC (Meliaceae). Jurnal Ilmiah Sains, 21(2), 161–165.
Liu, Ning, Xu, Hu, Sun, Qianqian, Yu, Xiaojuan, Chen, Wentong, Wei, Hongquan, Jiang, Jie, Xu, Youzhi, & Lu, Wenjie. (2021). The role of oxidative stress in hyperuricemia and xanthine oxidoreductase (XOR) inhibitors. Oxidative Medicine and Cellular Longevity, 2021.
Moonrungsee, Nuntaporn, Jakmunee, Jaroon, Peamaroon, Nipat, Boonmee, Apaporn, Kasemsuk, Teerapich, Seeda, Sureerat, & Suwancharoen, Sunisa. (2022). Phytochemical and Xanthine Oxidase Inhibitory Activity in Nypa fruticans Wurmb. Fruit Extracts. Trends in Sciences, 19(4), 2583.
Nugroho, Gilang D. W. I., Wiraatmaja, Muhammad Firdaus, Pramadaningtyas, Putri Segi, Febriyanti, Sarah, Liza, N. O. R., Naim, Darlina M. D., Ulumuddin, Yaya Ihya, & Setyawan, Ahmad D. W. I. (2020). Phytochemical composition, medicinal uses and other utilization of Nypa fruticans. International Journal of Bonorowo Wetlands, 10(1).
Owen, Patrick L., & Johns, Timothy. (1999). Xanthine oxidase inhibitory activity of northeastern North American plant remedies used for gout. Journal of Ethnopharmacology, 64(2), 149–160.
Praptiwi, Yuliasri Jamal, Keim, Ary P., & Agusta, Andria. (2015). Epikatekin Sebagai Komponen Kimia Utama Pada Daun Kayu Sina (Phyllocladus hypophyllus Hook. F.: Podocarpaceae). Prosiding Seminar Biodeversitas, 185–187.
Prawitasari, Dita Sukmaya. (2019). Diabetes melitus dan antioksidan. KELUWIH: Jurnal Kesehatan Dan Kedokteran, 1(1), 48–52.
Raharjo, Danang. (2022). Efektivitas Penghambatan Enzim Xantin Oksidase Ekstrak Etanol Dan Fraksi Etanol, Fraks Etil Asetat Serta Fraksi N-Heksane Kulit Batang Mangrove Merah (Rhizopora Mucronata). Jurnal Ilmiah Kesehatan, 15(1), 63–70.
Sekine, Mai, Okamoto, Ken, Pai, Emil F., Nagata, Koji, Ichida, Kimiyoshi, Hille, Russ, & Nishino, Takeshi. (2023). Allopurinol and oxypurinol differ in their strength and mechanisms of inhibition of xanthine oxidoreductase. Journal of Biological Chemistry, 299(9).
Solikhati, Devi Ika Kurnianingtyas, & Istriningsih, Endang. (2020). Aktivitas Antihiperuresemia Kombinasi Ekstrak Rimpang Lengkuas (Alpinia galanga L.) dan Allopurinol pada Mencit Putih Jantan Galur Swiss. Parapemikir: Jurnal Ilmiah Farmasi, 9(1), 38–45.
Tu, Lv Ying, Pi, Jiang, Jin, Hua, Cai, Ji Ye, & Deng, Sui Ping. (2016). Synthesis, characterization and anticancer activity of kaempferol-zinc (II) complex. Bioorganic & Medicinal Chemistry Letters, 26(11), 2730–2734.
Wei, Brian M., Fox, Lindy P., Kaffenberger, Benjamin H., Korman, Abraham M., Micheletti, Robert G., Mostaghimi, Arash, Noe, Megan H., Rosenbach, Misha, Shinkai, Kanade, & Kwah, Jason H. (2023). Drug-induced Hypersensitivity Syndrome/Drug Reaction with Eosinophilia and Systemic Symptoms. Part I. Epidemiology, Pathogenesis, Clinicopathological Features, and Prognosis. Journal of the American Academy of Dermatology.
Yuanta, Yohan. (2019). Pengaruh Pemberian Seduhan Rosella Terhadap Penurunan Kadar Asam Urat Wanita Menopause. ARTERI: Jurnal Ilmu Kesehatan, 1(1), 69–75.
Zahra, Tuffakhati Tsabitah. (2023). Studi Kasus Penurunan Kadar Asam Urat Pada Pasien Gout Arthritis Dengan Pemberian Jus Sirsak Ditatanan Keluarga. Repository Universitas Muhammadiyah Kendal Batang.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Jurnal Ilmiah Ibnu Sina
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
