AKTIVITAS ANTIBAKTERI DAN KOMPONEN KIMIA MINYAK ATSIRI TEMU KUNCI (Boesenbergia rotunda L. Mansf) TERHADAP BAKTERI PENYEBAB JERAWAT
DOI:
https://doi.org/10.36387/jiis.v9i2.1924Keywords:
acne, antibacterial activity, Boesenbergia rotunda, MIC, MBC, essential oilAbstract
Essential oil of fingerroot (Boesenbergia rotunda L. (Mansf)) have antibacterial activity against Staphylococcus but was not specific against acne-causing bacteria. The aim of this study was to determine its chemical content and antibacterial activity of the fingerroot oil against S. aureus, S. epidermidis, and Propionibacterium acnes that cause acne. The essential oil was isolated by steam and water distillation. The organoleptic, yield, and refractive index were observed. Gas chromatography-mass spectrometry (GC-MS) carried out the identification of essential oil components. Antibacterial activity of the oil was determined with the diffusion method. Broth microdilution methods was used for determination of the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC). Fingerroot essential oil was clear in color, aromatic odor, chelate taste, and slightly bitter with a yield of 1.19%. The refractive index was 1.4785 ± 0.0001. GC-MS identification showed 5 main components of fingerroot oil were camphor (28.70%), ocimene (24.71%), eucalyptol (16.26%), geraniol (14.81%), and methyl cinnamate (4.16%). The diameter of the zone of inhibition of bacterial growth was greatest at 20% concentration of fingerroot oil, followed by 10%, 5%, and 2.5%. MIC value of fingerroot oil against S. aureus and S. epidermidis was 0.625% and against P. acnes was 1.25%. The MBC value of fingerroot oil was doubled as the MIC value. The great antibacterial activity of fingerroot essential oil makes it potential to be developed into anti-acne preparations.
References
Afriyanti, R. N. (2015) ‘Akne Vulgaris Pada Remaja’, Medical Faculty of Lampung University, 4(6), pp. 102–109.
Apinundecha, C. et al. (2023) ‘Synergistic Interaction between Boesenbergia rotunda (L.) Mansf. Essential Oil and Cloxacillin on Methicillin-Resistant Staphylococcus aureus (MRSA) Inhibition’, Evidence-based Complementary and Alternative Medicine, 2023. doi: 10.1155/2023/3453273.
Baharudin, M. K. A., Hamid, S. A. and Susanti, D. (2015) ‘Chemical composition and antibacterial activity of essential oils from three aromatic plants of the zingiberaceae family in Malaysia’, Journal of Physical Science, 26(1), pp. 71–81.
Chahyadi, A. et al. (2014) ‘Boesenbergia Pandurata Roxb., An Indonesian Medicinal Plant: Phytochemistry, Biological Activity, Plant Biotechnology’, Procedia Chemistry. Elsevier Ltd., 13, pp. 13–37. doi: 10.1016/j.proche.2014.12.003.
Christiana, I. and Soegianto, L. (2020) ‘Skrining Senyawa Antibakteri dari Minyak Atsiri Rimpang Temu Kunci ( Boesenbergia pandurata ) terhadap Staphylococcus aureus dengan Metode Bioautografi Kontak’, Journal of Pharmacy Science And Practice, 7(1), pp. 15–19.
Depkes RI (2017) Farmakope Herbal Indonesia. II. Edited by D. K. Indonesia. Jakarta.
Eso, A., Mulyawati, S. and Rahmawati, E. (2020) ‘Uji Daya Hambat Fraksi N-Heksan dan Etil Asetat Rumput Laut Cokelat (Sargassum sp.) terhadap Pertumbuhan Bakteri Staphylococcus aureus (Inhibitory Effect of N-Hexane and Ethyl Acetate Fraction of Sargassum sp. Seaweeds against Staphylococcus aureus)’, Medula, 7(1), pp. 1–9. doi: 10.46496/medula.v7i1.11829.
Fardani, R. and Apriliani, R. (2023) ‘Uji Aktivitas Antibakteri Ekstrak Daun Suruhan (Peperomia Pellucida (L.) Kunth) Terhadap Bakteri Staphyloccocus Epidermidis’, JSN : Jurnal Sains Natural, 1(2), pp. 41–45. doi: 10.35746/jsn.v1i2.339.
Hafianty, F., Batubara, D. and Lingga, F. (2021) ‘Faktor risiko terjadinya akne vulgaris pada siswa - siswi kelas XII SMA harapan 1 medan’, Jurnal Ilmiah Simantek, 5(2), pp. 1689–1699.
Halvorsen, J. et al. (2011) ‘Suicidal ideation, mental health problems, and social impairment are increased in adolescents with acne: A population-based study.’, J Invest Dermatol., 131, pp. 363–70.
Hamzah, F. H. et al. (2023) ‘Physicochemical Characteristics and Anti-Bacterial Ability of Liquid Soap with the Addition of Pandan Wangi Extract’, Industria: Jurnal Teknologi dan Manajemen Agroindustri, 12(1), pp. 25–35. doi: 10.21776/ub.industria.2023.012.01.3.
Hasan, N., Mulyaningsih, S. and Setianto, A. (2024) ‘Aktivitas Antibakteri Nanoemulsi Kombinasi Minyak Sereh (Cymbopogon citratus (DC.) Stapf) dan Minyak Kemangi (Ocimum basilicum L.) terhadap Staphylococcus aureus’, Jurnal Sains dan Kesehatan, 6(1), pp. 62–73.
Hasrawati, A., Hasyim, N. and Irsyad, N. A. (2016) ‘Pengembangan Formulasi Mikroemulsi Minyak Sereh (Cymbopogon nardus) Menggunakan Emulgator Surfaktan Nonionik’, Jurnal Fitofarmaka Indonesia, 3(1), pp. 151–154. doi: 10.33096/jffi.v3i1.176.
Hay, R. J. et al. (2014) ‘The global burden of skin disease in 2010: An analysis of the prevalence and impact of skin conditions’, Journal of Investigative Dermatology. Nature Publishing Group, 134(6), pp. 1527–1534. doi: 10.1038/jid.2013.446.
Jantan, I. et al. (2001) ‘Constituents of the rhizome oils of Boesenbergia pandurata (Roxb.) Schlecht from Malaysia, Indonesia and Thailand’, Falavour and Fragrance Journal, 16(2), pp. 110–112.
Jitvaropas, R. et al. (2012) ‘Antioxidant, antimicrobial and wound healing activities of Boesenbergia rotunda’, Natural Product Communications, 7(7), pp. 909–912. doi: 10.1177/1934578x1200700727.
Kapoor, S. and Saraf, S. (2011) ‘Topical herbal therapies an alternative and complementary choice to combat acne’, Research Journal of Medicinal Plant, 5(6), pp. 650–669. doi: 10.3923/rjmp.2011.650.669.
McLaughlin, J. et al. (2019) ‘Propionibacterium acnes and acne vulgaris: New insights from the integration of population genetic, multi-omic, biochemical and host-microbe studies’, Microorganisms, 7(5). doi: 10.3390/microorganisms7050128.
Miksusanti et al. (2008) ‘Kerusakan Dinding Sel Escherechia coli Kl.l OLEH Minyak Atsiri Temu Kunci (Kaemferiapandurata)’, Berita Biologi, 9, pp. 1–8.
Mulyaningsih, S., Ramadhan, A. G. and Putranti, W. (2023) ‘Antibacterial Effect of Cinnamon and Citronella Oils Combination Against Acne-Related Bacteria’, Borneo Journal of Pharmacy, 6(3), pp. 305–313. doi: 10.33084/bjop.v6i3.4735.
Putranti, W. and Bachri, S. (2018) ‘Uji toksisitas minyak atsiri rimpang temu kunci (Boesenbergia pandurata (Roxb) Schlecht) terhadap larva Aedes aegypti serta profil GC-MS’, Traditional Medicine Journal, 23(2), pp. 97–102.
Rahman, M., Yan, L. and Rukayadi, Y. (2016) ‘Antibacterial activity of fingerroot (Boesenbergia rotunda) extract against acne-inducing bacteria’, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(6), pp. 2157–2163.
Veerasophon, J., Sripalakit, P. and Saraphanchotiwitthaya, A. (2020) ‘Formulation of anti-acne concealer containing cinnamon oil with antimicrobial activity against Propionibacterium acnes’, Journal of Advanced Pharmaceutical Technology and Research, 11(2), pp. 53–58. doi: 10.4103/japtr.JAPTR_1_20.
Xavier-Junior, F. H. et al. (2017) ‘Microemulsion systems containing bioactive natural oils: an overview on the state of the art’, Drug Development and Industrial Pharmacy, 43(5), pp. 700–714. doi: 10.1080/03639045.2016.1235186.
Zainita, R. (2022) Aktivitas Antijamur Kombinasi Minyak Atsiri Rimpang Temu Kunci (Boesenbergia pandurata) dan Minyak Atsiri Peppermint (Mentha pipperita) Pada Candida albicans. Universitas Sriwijaya.
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