AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM DARI EKSTRAK DAUN BANGUN-BANGUN (Coleus amboinicus L.) TERHADAP BAKTERI Staphylococcus aureus

Authors

  • Fitra Romadhonsyah Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta https://orcid.org/0000-0001-9686-3289
  • Aisha Nanda Caela Amry Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Annisa Fitria Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Arde Toga Nugraha Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Arba Pramundita Ramadani Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Nangim Khasanah Laboratorium Mikrobiologi, Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta

DOI:

https://doi.org/10.36387/jiis.v9i2.2173

Keywords:

antibacterial, antibiofilm, Coleus amboinicus, Staphylococcus aureus

Abstract

Biofilm formation by bacteria, especially the genus Staphylococcus, is a serious health problem. Various medicinal plants have been used to control the infection, but research related to the antibiofilm activity of Coleus amboinicus is still limited. Bangun-bangun leaves (C. amboinicus) are plants from the Lamiaceae family that contain secondary metabolites such as terpenoids, flavonoids, and phenolics that are thought to be responsible as antibacterial and antibiofilm. This study aims to examine the antibacterial and antibiofilm activity of C. amboinicus against S. aureus. C. amboinicus leaves were extracted using 96% ethanol. The ethanol extract was then screened for phytochemicals using Thin Layer Chromatography (TLC) using several spray reagents. Antibacterial activity was analyzed using microdilution technique to obtain the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Antibiofilm activity was analyzed using crystal violet method. The result showed that the ethanol extract of C. amboinicus leaves contained flavonoids, phenolic terpenoids, and tannins. Antibacterial assays were obtained for the MIC value of 8 mg/mL and MBC of 16 mg/mL. The ethanol extract showed biofilm inhibitory activity with an IC50 value of 2,40 mg/mL. Based on these results, C. amboinicus has potential antibacterial and antibiofilm activity to inhibit S. aureus.

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Published

2024-11-26

Issue

Vol. 9 No. 2 (2024): JIIS

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Article

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How to Cite

AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM DARI EKSTRAK DAUN BANGUN-BANGUN (Coleus amboinicus L.) TERHADAP BAKTERI Staphylococcus aureus. (2024). JIIS (Jurnal Ilmiah Ibnu Sina): Ilmu Farmasi Dan Kesehatan, 9(2), 419-430. https://doi.org/10.36387/jiis.v9i2.2173

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