UJI TOKSISITAS KITOSAN CANGKANG KERANG BULU (Anadara antiquata) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

  • Nadila Indra Sepvina Program Studi Farmasi, Universitas Muslim Nusantara Al Washliyah
  • Ridwanto Ridwanto Program Studi Farmasi, Universitas Muslim Nusantara Al Washliyah
  • Zulmai Rani Program Studi Farmasi, Universitas Muslim Nusantara Al Washliyah
Keywords: Shellfish, Chitosan, Toxicity test, BSLT

Abstract

Shellfish is one of Indonesia's fishery commodities which is increasing every year, one of which is blood clams and feather shells, which are easily found in Southeast Asia and East Asia. Shellfish also have great potential so that it has an impact on the increasing waste that is allowed to accumulate without any handling of the pollution that exists in the environment and society. One alternative to the use of waste from feather shells to have a high use value is to isolate it into chitosan. The purpose of this study was to investigate the utilization of shellfish waste into chitosan and to determine the LC50 value of the chitosan produced. The analysis carried out was the toxicity test of chitosan from shellfish shell waste on Artemia salina Leach shrimp larvae using the Brine Shrimp Lethalitiy Test (BSLT) method with various concentrations of 100; 250; 500; 750; and 1000 µg/mL, and determining the value of LC50 by probit analysis. Based on the results of the toxicity test of chitosan from shellfish waste, the LC50 value was µg/mL. These results indicate that chitosan from shellfish waste has no toxic effect on Artemia salina Leach shrimp larvae.

References

Andini, A., Prayekti, E., Triasmoro, F., & Kamaliyah, I. N. 2021. Pengaruh Penggunaan Jenis Pelarut dalam Uji Sitotoksistas Metode Brine Shrimp Lethality Test (BSLT) pada Wound Dressing Kolagen-Kitosan. Al-Kimiya, 8(1), 15–20.

Ariyanti, A., Masruriati, E., Imadahidayah, T., & Sulistia ningsih, E. N. 2020. Pemanfaatan kitosan dari cangkang kerang bulu (Anadara antiquata) sebagai pengawet ikan pari (Dasyatis sp.) dan udang vaname (Litopenaeus vannamei). Riset Informasi Kesehatan, 9(1), 12.

Djohari, M., Efendi, T. T., Rahmawati, N., Husnawati, & Aryani, F. 2022. Uji Toksisitas Akut Dan Tertunda Fraksi Butanol Daun Akar Kaik-Kaik (Uncaria cordata (Lour) Merr.) Pada Mencit Putih (Mus musculus L.). Jurnal Ilmiah Ibnu Sina, 203-211.

Ersalina, N., & Ary, A. 2021. Uji Toksisitas Dengan Metode BSLT Pada Nanopartikel Kombinasi Obat Cisplatin, Bovine Serum Albumin Dan Asam Folat. Jurnal Ilmiah Ibnu Sina, 69-78.

Fauziah, Y., Setiawan, M. A., & Fitriyani. 2018. Uji Daya Hambat Ekstrak Kerang Tahu (Meretrix meretrix) Terhadap Bakteri Staphylococcus aureus. Jurnal Ilmiah Ibnu Sina, 19-27.

Masindi, T., & Herdyastuti, N. 2017. Karakterisasi Kitosan Dari Cangkang Kerang Darah (Anadara granosa). Journal of Chemistry, 6 (3), 137–142.

Masruriati, E. 2019 .Perbandingan Pengawet Kitosan Dari Cangkang Kerang Bulu (Anadara antiquata) Sebagai Pengawet Udang Vaname (Litopenaeus vannamei) Dan Ikan Pari (Dasyatis sp.). Jurnal Farmasi (Journal of Pharmacy), 1 (1), 23–28.

Musiam, S., & Aisyah, N. 2019. Sintesis Nanokitosan Dari Limbah Cangkang Haliling (Filopaludina javanica) Kalimantan Selatan. Jurnal Ilmiah Ibnu Sina , 432-439.

Nur, N., Windari, R., Fauziah, S. I., Juniar, A. E., & Purnomo, T. (2019). Biobakterisida Kitosan Cangkang Kerang Darah sebagai Anti Bakteri Ralstonia solanacearum Biobactericide Chitosan Blood Shell as an Anti Bacterial Ralstonia solanacearum. Microbiology, 16, 280–284.

Nurhayati, A. P. D., Abdulgani, N., & Febrianto, R. 2006. Uji Toksiistas Ekstrak Eucheuma alvarezii terhadap Artemia salina Leach Studi Pendahulan Potensi Antikanker. Akta Kimindo, 2 (1), 41–46.

Pradana, A. T., Nawatila, R., & Rachman, M. R. 2022. Karakteristik Fisik Mikropartikel Kuersetin Dengan Kombinasi Kitosan-Natrium Tripolifosfat Menggunakan Metode Orifice Ionic Gelation. Jurnal Ilmiah Ibnu Sina , 133-142.

Rani, Z, Ridwanto , Dikki, M., Rafita, Y., Ani, S., Ricky, A, S., and Reza, I., 2022. “Cytotoxicity Test of Cocoa Leaf Ethanol Ex-tract (Theobroma Cacao L.) With Brine Shrimp Lethality Test (BSLT) Method.†Indo-nesian Journal of Chemical Science and Technology (IJCST) 5 (2): 80–87.

Ridwanto., Gabena, I, D., Anny, S, D. 2020. Isolasi Dan Penggunaan Kitosan Alami Sebagai Pengawet Buah Strawberry. Prosiding Seminar Nasional Hasil Penelitian UMN AW. 404–503.

Ridwanto, Daulay, A. S., & Gurning, K. 2022. Isolation and Characterization of Chitosan From Sea and Freshwater Waste, North Sumatera Province, Indonesia. Rasayan Journal of Chemistry, 15(2), 780–785.

Saragih, D. S. et al. 2022. Toxicity Test of Windu Shrimp (Penaeus monodon) Skin Chitosan With Brine Shrimp Lethality Test Method. Indonesian Journal of Chemical Science and Techonology, 05 (1), pp. 4–6.

Silaban, R., Silubun, D. T., & Jamlean, A. A. R. 2021. Aspek Ekologi Dan Pertumbuhan Kerang Bulu (Anadara antiquata) Di Perairan Letman, Kabupaten Maluku Tenggara. Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 14 (2), 120–131.

Published
2022-10-31
How to Cite
Sepvina, N., Ridwanto, R., & Rani, Z. (2022). UJI TOKSISITAS KITOSAN CANGKANG KERANG BULU (Anadara antiquata) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT). Jurnal Ilmiah Ibnu Sina, 7(2), 380-389. https://doi.org/10.36387/jiis.v7i2.1023
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