OPTIMASI FASE GERAK PADA KLT DAN IDENTIFIKASI METABOLIT SEKUNDER Ipomoea pes-caprae
DOI:
https://doi.org/10.36387/jiis.v11i1.2995Keywords:
Ipomoea pes-caprae , TLC, Optimization , Mobile Phase, Secondary MetabolitesAbstract
Ipomoea pes-caprae (L.) R.Br traditionally used to treat pain, skin infections, inflammation, and rheumatic disorders. In vitro studies also reported antioxidant, anti-inflammatory, and antimicrobial potential activities obtained from secondary metabolites such as flavonoids, tannins, terpenoids, steroids, saponins, and alkaloids contained in Ipomoea pes-caprae. The purpose of this study is to optimize the mobile phase and identify secondary metabolite compounds of Ipomoea pes-caprae. The analysis used thin layer chromatography (TLC) in identifying compounds responsible for providing antioxidant activity with a spray reagent in the form of DPPH. The results showed the ideal of mobile phase, namely Chloroform: Ethyl Acetate: Methanol: Formic Acid (2: 7: 0.5: 0.5). Ethanol extract of 96% leaves has antioxidant activity with a DPPH spray reaction than ethyl acetate and N-hexane extracts. The metabolite profiles in samples with various specific reagents include the group of polyphenols, alkaloids and flavonoid compounds. Based on the results of the descriptive analysis, it was shown that the most dominant group of flavonoid compounds from ethanol extract was 96% of Ipomoea pes-caprae leaves, so it had a strong correlation with antioxidant activity.
References
Abubakar, A. R., & Haque, M. (2020). Preparation Of Medicinal Plants: Basic Extraction And Fractionation Procedures For Experimental Purposes. Journal Of Pharmacy And Bioallied Sciences, 1–10. Https://Doi.Org/10.4103/Jpbs.JPBS
Agati, G., Azzarello, E., Pollastri, S., & Tattini, M. (2012). Flavonoids As Antioxidants In Plants: Location And Functional Significance. Plant Science, 196, 67–76. Https://Doi.Org/10.1016/J.Plantsci.2012.07.014
Akinniyi, G., Lee, J., Kim, H., Lee, J.-G., & Yang, I. (2022). A Medicinal Halophyte Ipomoea Pes-Caprae ( Linn .) R . Br .: A Review Of Its Botany, Traditional Uses, Phytochemistry, And Bioactivity Ganiyu. Marine Drugs.
Alagesan, V., Ramalingam, S., Kim, M., & Venugopal, S. (2019). Antioxidant Activity Guided Isolation Of A Coumarin Compound From Ipomoea Pes-Caprea ( Convolvulaceae ) Leaves Acetone Extract And Its Biological And Molecular Docking Studies. European Journal Of Integrative Medicine, 32(October), 100984. Https://Doi.Org/10.1016/J.Eujim.2019.100984
Ariffly, N. ‘Aisyah T., Abdullah, T. A., Mohd, K. S., Bakar, F. A., & Alwi, A. (2025). Phytochemical Screening And Antioxidant Properties Of Ficus Carica L . Leaves Fig Leaves Were Also Discovered To Possess A Significant Quantity Of Phenolic Compounds And Good Antioxidant Capacities ( Magalhes Et Al ., 2017 ). In Addition , A Research Pape. MALAYSIAN JOURNAL OF APPLIED SCIENCES, 10, 56–64.
Arifuddin, M., & Bone, M. (2020). Skrining Fitokimia Dan Profil Kromatografi Lapis Tipis (KLT) Tumbuhan Antimalaria Asal Indonesia M. Jurnal Sains Dan Kesehatan, 3(3), 174–181.
Attia, K. A. M., Desouky, E. A. El, Abdelfatah, A. M., & Abdelshafi, N. A. (2023). Simultaneous Analysis Of The Of Levamisole With Triclabendazole In Pharmaceuticals Through Developing TLC And HPLC – PDA Chromatographic Techniques And Their Greenness Assessment Using GAPI And AGREE Methods. BMC Chemistry, 1–15. Https://Doi.Org/10.1186/S13065-023-01087-X
Boyd, D. B., Mungmai, L., Vimolmangkang, S., & Uputinan, S. (2025). TLC-Bioautography-Guided Valorization Of Vetiver ( Chrysopogon Spp .) Leaf Extracts Into An Anti-Acne Gel. Bioresources And Bioprocessing, 1–16.
Candra, H., Oktaviani, F., & Putri, A. (2025). Cream Formulation Of Methanol Extract Of Tapak Kuda Leaves ( Ipomoea Pes Caprae ( L .) R . Br .) As Anti-Inflammatory In Male White Mice ( Mus Musculus ). International Journal Of Health And Medicine, 1.
Denni, M., & Mammen, D. (2012). A Critical Evaluation On The Reliability Of Two Aluminum Chloride Chelation Methods For Quantification Of Flavonoids. Food Chemistry, 135, 1365–1368.
Dewati, P. R., Rochmadi, R., Rohman, A., Darwito, D., & Budiman, A. (2024). Determination Of The Mobile Phase In Low-Pressure Column Chromatography Using Thin-Layer Chromatography To Purify Astaxanthin. AIP Conference Proceedings.
Eric, T. G., Edith, K. A. A., Merveille, K. G., Donald, K., & Gisèle, K.-S. N. (2025). In Vitro Antioxidant Activity, Physicochemical And Micrographic Studies Of The Leafy Stem Of Ipomoea Pes-Caprae (L) R. Br, A Halophyte From Ivory Coast. Imternational Journal Of Advanced Research, 13(01), 102–111. Https://Doi.Org/10.21474/IJAR01/20169
Ferraro, A., Guzzi, R., Sighano, S. K., Nicoletta, G., Caputo, R., Cofone, F., Desiderio, G., & Gennari, O. (2025). Optically Active , Chlorophyll-Based Fluorescent Dye From Calabrian Opuntia Ficus-Indica Cladodes For Sustainable Applications. 1–22.
Giri, S., Giri, U., Subedi, K., Magar, K. T., Pant, S., & Joshi, K. R. (2020). Thin Layer Chromatography Based Chemical Profiling And Antioxidant Activity Of Selected Nepalese Medicinal Plants. JHAS, 10(2), 15–22.
Grotewold, E. (2006). The Genetics And Biochemistry Of Floral Pigments. Annual Review Of Plant Biology, 57, 761–780. Https://Doi.Org/10.1146/Annurev.Arplant.57.032905.105248
Herwin, Baits, M., & Ririn. (2015). Aktivitas Antibakteri Dan Antioksidan Fraksi Daun Colocacia Esculenta L. Dengan Metode Klt-Bioautografi Dan Difenilpikril Hidrazil. As-Syifaa Jurnal Farmasi, 07(02).
Hikma, N. A., Rahman, A. N. A., Basir, S. A., Yeyen, A., Syam, A. P. W., & S, M. H. (2025). Isolasi Dan Identifikasi Senyawa Metabolit Sekunder Ekstrak Daun Kopi Arabika ( Coffea Arabica ) Menggunakan Teknik Kromatografi. Inovasi Kesehatan Global, 2(3), 198–209.
Hunaifi, I., Suryani, D., Hidayat, L. H., Naufal, M., Ananta, F., & Farobbi, M. I. (2025). Preliminary In Vitro Antiplatelet Potential Of Ipomoea Pes-Caprae From North Lombok With Adenosine Diphosphate-Induced Platelet Aggregation. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 12(2), 228–245. Https://Doi.Org/10.20473/Jfiki.V12i22025.228-245
Ivanova, S., Todorova, V., Dyankov, S., & Ivanov, K. (2022). High-Performance Thin-Layer Chromatography (HPTLC) Method For Identification Of Meloxicam And Piroxicam. Processes.
Kemenkes RI. (2017). FARMAKOPE HERBAL INDONESIA ED. II. In Jakarta: Departement Kesehatan Republik Indonesia. Https://Doi.Org/10.2307/Jj.2430657.12
Laferriere-Holloway, T. S., Rios, A., Lu, Y., Okoro, C. C., & Dam, R. M. Van. (2024). A Rapid And Systematic Approach For The Optimization Of Radio-TLC Resolution. HHS Public Access. Https://Doi.Org/10.1016/J.Chroma.2022.463656.A
Mayasari, D., Murti, Y. B., Pratiwi, S. U. T., & Sudarsono, S. (2022). Antibacterial Activity And TLC-Densitometric Analysis Of Secondary Metabolites In The Leaves Of The Traditional Herb, Melastoma Malabathricum L. Borneo Journal Of Pharmacy, 5(4), 334–344. Https://Doi.Org/10.33084/Bjop.V5i4.3818
Mulangsri, D. A. K., Hidayati, D. N., & Puspitasari, A. (2024). Penyuluhan Dan Praktik Pengelolaan Pascapanen Tanaman Obat Di Plalangan Gunungpati Semarang. Jurnal Ilmiah Pengabdian Kepada Masyarakat, 9(1), 140–147.
Munawir, Harmoni, D. N. S., & Alfarizi, L. M. (2025). Isolasi Dan Penetapan Kadar Senyawa Flavonoid Pada Daun Pepaya (Carica Papaya L.) Menggunakan Metode KLT Dan Spektrofotometri UV-Vis. Jurnal Ilmiah Kesehatan.
Murugesan, M., Kandhavelu, M., Thiyagarajan, R., Natesan, S., Rajendran, P., & Murugesan, A. (2023). Biomedicine & Pharmacotherapy Marine Halophyte Derived Polyphenols Inhibit Glioma Cell Growth Through Mitogen-Activated Protein Kinase Signaling Pathway. Biomedicine & Pharmacotherapy, 159, 114288. Https://Doi.Org/10.1016/J.Biopha.2023.114288
Muthia, R., Hidayatullah, M., & Hidayati, R. (2020). Phytochemical Screening And Antioxidant Activity Of Ethanolic Extract Of Cawat Hanoman Stem ( Bauhinia Aculeata L .) Using DPPH Method. Borneo Journal Of Pharmacy, 3(1), 15–21.
Nuskiya, A., Pringgenies, D., & Trianto, A. (2025). Skrining Fitokimia Dan Evaluasi Antioksidan Serta Antibakteri Ekstrak Mangrove Asal Pantai Lampu Satu. Jurnal Kelautan Tropis, 28(November), 521–528.
Nuskiya, A., Sibero, M. T., Setyati, W. A., Andriani, C., Hendryanti, D. N., & 1. (2022). Bioprospecting Of Katang-Katang Leaves (Ipomoea Pes-Caprae) From Sumba Island, East Nusa Tenggara: Antimicrobial, Antioxidant And Secondary Metabolites Content. IOP Publishing. Https://Doi.Org/10.1088/1755-1315/1260/1/012054
Oktavianawati, I., Santoso, M., & Fatmawati, S. (2023). Metabolite Profiling Of Borneo ’ S Gonystylus Bancanus Through Comprehensive Extraction From Various Polarity Of Solvents. Scientific Reports, 0123456789, 1–19. Https://Doi.Org/10.1038/S41598-023-41494-7
Prasetyawan, H. R., Primaharinastiti, I., & Riesta, K. (2024). Teknik Aplikasi Sampel Pada Pengujian Kuantitatif Kromatografi Lapis Tipis: Tinjauan Terhadap Area Dan Faktor Retensi. Media Farmasi, 20(2).
Raj, D. (2022). Eutectic Thin-Layer Chromatography As A New Possibility For Quantification Of Plant Extracts—A Case Study. Molecules, 27, 2960.
Segiawati, A., Ensu, E., Said, M., Yeyen, Y., & Leto, K. T. (2025). Skrining Fitokimia Daun Katang – Katang ( Ipomoea Pescaprae L . ). Jurnal Matematika Dan Ilmu Pengelatuan Alam, 3.
Sembiring, B. B., Fulazzaky, M. A., Haris, H., Rachmat, R., Histifarina, D., Siregar, T. H., & Triyono, A. (2025). Quality Of Sengkubak Leaf Simplicia From Various Stages Of Maturity And Drying Methods. 2061, 1–11.
Siskayanti, R., Rizkiah, R., Muliati, L., Nurilah, A., Subagja, D., & Fadil, M. I. (2024). Extraction Of Polyphenols In Green Tea Shoots As Antioxidant Substance. Jurnal Sain Dan Teknik, 6(199).
Sumardi, Zebua, N. F., Mierza, V., Arafah, R. M., & Amalia, R. (2023). Profil Noda Ekstrak Kloroform : Metanol ( 2 : 1 ) Dan Aktivitas Bslt Kulit Batang Nyirih ( Xylocarpus Granatum ) Journal Of Pharmaceutical And Health Research. Journal Of Pharmaceutical And Health Research, 4(2), 326–332. Https://Doi.Org/10.47065/Jharma.V4i2.3678
Wildani, W., Malem, R., Tanjung, W. F., & Abdiansyah, A. (2022). Skrining Fitokimia Dan Aktivitas Antibakteri Fraksi N- Heksan Ekstrak Metanol Daun Kerai Payung (Filicium decipiens) Terhadap Staphylococcus epidermidis. Pharmaceutical Journal of Islamic Pharmacy, 6(1), 1–11. https://doi.org/10.21111/pharmasipha.v5i1
Zhong, F., Li, S., Pan, X., Wen, J., Xie, J., Du, Z., Hao, E., Deng, J., & Hou, X. (2025). Investigating the Mechanism of Action of Ipomoea pes-caprae in the Treatment of Rheumatoid Arthritis Based on Serum Metabolomics and Network Pharmacology. Marine Drugs, 23, 114. https://doi.org/https://doi.org/10.3390/md23030114
Zoremsiami, J., & Gc, J. (2018). The Phytochemical and Thin Layer Chromatography Profile of Ethnomedcinal Plant Helicia Nilagirica ( Bedd ). International Journal of Pharmacacognosy and Chinese Medicine, 2(2).
Zych, M., & Pyka-paj, A. (2025). TLC in the Analysis of Plant Material. Processes, 1–47.
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