UJI AKTIVITAS OBAT HERBAL GEL EKSTRAK DAUN GAHARU (Gyrinops versteegii (Gilg.) Domke) TERHADAP LUKA DIABETES PADA TIKUS PUTIH JANTAN (Rattus norvegicus)

Abstract

A study to test the activity of gel extract of gaharu (Gyrinops versteegii (Gilg.) Domke) leaf extract against diabetic wounds in male white rats (Rattus norvegicus). The research was conducted experimentally with the method of pre-test and post-test with group design. Gaharu leaf extract gel was formulated with concentrations of 4%, 6% and 8% and then tested for its activity on non-diabetic male white rats and diabetic male rats with alloxan induction. The negative control was given gel base and the positive control was given bioplacenton gel. The gel was administered once a day and the wound length was measured every day for 14 days. The results showed that all gel formulas of gaharu leaf extract had wound healing activity in non-diabetic rats and diabetic rats, the higher the concentration of the extract, the faster the wound healing and the greater the percentage reduction in wound length. The group of non-diabetic rats treated with gaharu extract gel with a concentration of 8% showed a wound length of 0,00 ± 0,00 mm (wound healed) on day 6 with an average percentage reduction in wound length of 100,00 ± 0,00 %. The results of statistical testing with Kruskal Wallis obtained p-value > 0.05 (p-value = 0.315) so it can be concluded that there was no significant difference between the treatment groups.

References

Anggraeni, D., Airin, C. M., & Raharjo, S. (2018). The Effectiveness Of Ethanol Extract Of Binahong Leaves On Diabetic Wound Healing. Jurnal Kedokteran Hewan - Indonesian Journal Of Veterinary Sciences, 11(4). Https://Doi.Org/10.21157/J.Ked.Hewan.V11i4.6562

Dirga, D., Khairunnisa, S. M., Akhmad, A. D., Setyawan, I. A., & Pratama, A. (2021). Evaluasi Penggunaan Antibiotik Pada Pasien Rawat Inap Di Bangsal Penyakit Dalam Rsud. Dr. H. Abdul Moeloek Provinsi Lampung. Jurnal Kefarmasian Indonesia, 65–75. Https://Doi.Org/10.22435/Jki.V11i1.3570

Harbone, J. B. (1987). Metode Fitokimia; Penuntutan Cara Modern Menganalisa Tumbuhan (Ii). Itb Bandung.

I G. A. A. D, Y., D.A, S., & I M, K. (2013). Skrining Fitokimia Ekstrak Metanol Daun Gaharu (Gyrinops Versteegii (Gilg) Domke). Jurnal Farmasi Udayana, 2. Https://Ojs.Unud.Ac.Id/Index.Php/Jfu/Article/View/7403

Pertiwi, R., Manaf, S., Supriati, R., Saputra, H. M., & Ramadhanti, F. (2020). Pengaruh Pemberian Salep Kombinasi Ekstrak Daun Morinda Citrifolia Dan Batang Euphorbia Tirucalli Terhadap Penyembuhan Luka. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 7(1), 42. Https://Doi.Org/10.20473/Jfiki.V7i12020.42-50

Riani, N., Kep, S., Kes, M., Handayani, F., & Kes, M. (2017). Perbandingan Efektivitas Perawatan Luka Modern “Moist Wound Healing” Dan Terapi Komplementer “Nacl 0,9% + Madu Asli” Terhadap Penyembuhan Luka Kaki Diabetik Derajat Ii Di Rsud Bangkinang. 1(2), 98–107.

Suhardiman, A., & Juanda, D. (2019). Pengembangan Obat Herbal Fraksi Daun Gaharu (Aquilaria Malaccensis Lam)Dalam Bentuk Gel Untuk Penyembuhan Luka Bakar. Jurnal Sains Dan Teknologi Farmasi Indonesia, Viii, 16–26.

Suriadi, S. (2004). Perawatan Luka (1st Ed., Vol. 1). Sagung Seto.

Swastini, D. A., Shaswati, G. A. P. A., Widnyana, I. P. S., Amin, A., Kusuma, L. A. S., Putra, A. A. R. Y., & Samirana, P. O. (2018). Penurunan Kadar Glukosa Darah Dan Gambaran Histopatologi Pankreas Dengan Pemberian Gula Aren (Arenga Pinnata) Pada Tikus Jantan Galur Wistar Yang Diinduksi Aloksan. Indonesia Medicus Veterinus, 10. Https://Doi.Org/10.19087/Imv.2018.7.2.94

Tampala, B. (2018). Uji Efektivitas Ekstrak Etanolik Batang. Jurnal Ilmiah Ibnu Sina, 3(2), 318–327. Https://Doi.Org/10.36387/Jiis.V3i2.185

Tulung, G. L., Bodhi, W., & Siampa, J. P. (2021). Uji Efektivitas Ekstrak Etanol Daun Pegagan (Centella Asiatica (L.) Urban) Sebagai Antidiabetes Terhadap Tikus Putih Jantan (Rattus Norvegicus) Yang Diinduksi Aloksan. Pharmacon, 10(1), 736. Https://Doi.Org/10.35799/Pha.10.2021.32767

Yang, X.-B., Feng, J., Yang, X.-W., Zhao, B., & Liu, J.-X. (2012). Aquisiflavoside, A New Nitric Oxide Production Inhibitor From The Leaves Of Aquilaria Sinensis. Journal Of Asian Natural Products Research, 14(9), 867–872. Https://Doi.Org/10.1080/10286020.2012.701209