AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM DARI EKSTRAK DAUN BANGUN-BANGUN (Coleus amboinicus L.) TERHADAP BAKTERI Staphylococcus aureus

Authors

  • Fitra Romadhonsyah Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta https://orcid.org/0000-0001-9686-3289
  • Aisha Nanda Caela Amry Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Annisa Fitria Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Arde Toga Nugraha Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Arba Pramundita Ramadani Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta
  • Nangim Khasanah Laboratorium Mikrobiologi, Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia, Yogyakarta

DOI:

https://doi.org/10.36387/jiis.v9i2.2173

Keywords:

antibacterial, antibiofilm, Coleus amboinicus, Staphylococcus aureus

Abstract

Biofilm formation by bacteria, especially the genus Staphylococcus, is a serious health problem. Various medicinal plants have been used to control the infection, but research related to the antibiofilm activity of Coleus amboinicus is still limited. Bangun-bangun leaves (C. amboinicus) are plants from the Lamiaceae family that contain secondary metabolites such as terpenoids, flavonoids, and phenolics that are thought to be responsible as antibacterial and antibiofilm. This study aims to examine the antibacterial and antibiofilm activity of C. amboinicus against S. aureus. C. amboinicus leaves were extracted using 96% ethanol. The ethanol extract was then screened for phytochemicals using Thin Layer Chromatography (TLC) using several spray reagents. Antibacterial activity was analyzed using microdilution technique to obtain the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Antibiofilm activity was analyzed using crystal violet method. The result showed that the ethanol extract of C. amboinicus leaves contained flavonoids, phenolic terpenoids, and tannins. Antibacterial assays were obtained for the MIC value of 8 mg/mL and MBC of 16 mg/mL. The ethanol extract showed biofilm inhibitory activity with an IC50 value of 2,40 mg/mL. Based on these results, C. amboinicus has potential antibacterial and antibiofilm activity to inhibit S. aureus.

References

Arumugam, G., Swamy, M., & Sinniah, U. (2016). Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules, 21(4), 369. https://doi.org/10.3390/molecules21040369

Dwivedi, D., & Singh, V. (2016). Effects of the natural compounds embelin and piperine on the biofilm-producing property of Streptococcus mutans. Journal of Traditional and Complementary Medicine, 6(1), 57–61. https://doi.org/10.1016/j.jtcme.2014.11.025

Endarini, L. H. (2019). Analisis Rendemen Dan Penetapan Kandungan Ekstrak Etanol 96% Daun Teh Hijau (Camellia sinensis L.,) Dengan Metode Kromatografi Lapis Tipis. Prosiding dari Seminar “SEMNASKes -2019 ‘Improving The Quality of Health Through Advances in Research of Health Sciences,’” 30–40.

Faleye, O. S., Lee, J.-H., & Lee, J. (2023). Selected flavonoids exhibit antibiofilm and antibacterial effects against Vibrio by disrupting membrane integrity, virulence and metabolic activities. Biofilm, 6, 100165. https://doi.org/10.1016/j.bioflm.2023.100165

Fitria, A. (2018). The Bactericidal and Antibiofilm Activity of Stem Bark of Jatropha multifida L. Against Staphylococcus aureus and MRSA. EKSAKTA: Journal of Sciences and Data Analysis, 42–55. https://doi.org/10.20885/eksakta.vol18.iss1.art5

Leesombun, A., Sungpradit, S., Sariya, L., Taowan, J., & Boonmasawai, S. (2023). Transcriptional Profiling of the Effect of Coleus amboinicus L. Essential Oil against Salmonella Typhimurium Biofilm Formation. Antibiotics, 12(11), 1598. https://doi.org/10.3390/antibiotics12111598

Luqman, A., Alami, N. H., Dama, B. F., Auchenfloretta, B., & Danilyan, E. (2021). Bakteriologi Spesies Kosmopolit. Departemen Biologi Institut Teknologi Sepuluh November. https://www.researchgate.net/publication/354492714_BAKTERIOLOGI_SPESIES_KOSMOPOLIT

Mirghani, R., Saba, T., Khaliq, H., Mitchell, J., Do, L., Chambi, L., Diaz, K., Kennedy, T., Alkassab, K., Huynh, T., Elmi, M., Martinez, J., Sawan, S., & Rijal, G. (2022). Biofilms: Formation, drug resistance and alternatives to conventional approaches. AIMS Microbiology, 8(3), 239–277. https://doi.org/10.3934/microbiol.2022019

Ocktaviana Saputri, L., Octora, M., Ferdiana, A., Andiwijaya, F., Hasbi, N., & Rafiq, A. (2022). Program Pengendalian Resistensi Antibiotik Di Tengah Pandemik Covid-19 Bagi Tenaga Kesehatan Di Indonesia. Jurnal Abdi Insani, 9(4), 1780–1788. https://doi.org/10.29303/abdiinsani.v9i4.781

Rollando, R. (2019). Uji Antimikroba Minyak Atsiri Masoyi (Massoia aromatica) Terhadap Bakteri Streptococcus mutans. Majalah Farmasi dan Farmakologi, 23(2), 52–57. https://doi.org/10.20956/mff.v23i2.6585

Romadhonsyah, F., Gemantari, B. M., Nurrochmad, A., Wahyuono, S., & Astuti, P. (2022). Antioxidant, Cytotoxic Activities and Characterization of Secondary Metabolites of Endophytic Fungus Schizophyllum commune isolated from Coleus amboinicus (Lour.) Leaves. Research Journal of Pharmacy and Technology, 15(1), 357–364. https://doi.org/10.52711/0974-360X.2022.00058

Shree, P., Singh, C. K., Sodhi, K. K., Surya, J. N., & Singh, D. K. (2023). Biofilms: Understanding the structure and contribution towards bacterial resistance in antibiotics. Medicine in Microecology, 16, 100084. https://doi.org/10.1016/j.medmic.2023.100084

Shubha, J. R., & Bhatt, P. (2015). Plectranthus amboinicus leaves stimulate growth of probiotic L. plantarum: Evidence for ethnobotanical use in diarrhea. Journal of Ethnopharmacology, 166, 220–227. https://doi.org/10.1016/j.jep.2015.02.055

Sivaranjani, D., Saranraj, P., Manigandan, M., & Amala, K. (2019). Antimicrobial activity of Plectranthus amboinicus solvent extracts against Human Pathogenic Bacteria and Fungi. Journal of Drug Delivery and Therapeutics, 9(3), 36–39. https://doi.org/10.22270/jddt.v9i3.2604

Swamy, M., Arumugam, G., Kaur, R., Ghasemzadeh, A., Yusoff, M., & Sinniah, U. (2017). GC-MS Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus amboinicus Leaves. Evidence-Based Complementary and Alternative Medicine, 2017, 1–10. https://doi.org/10.1155/2017/1517683

Syarifuddin, A., & Sulistyani, N. (2019). Karakterisasi Fraksi Teraktif Senyawa Antibiotik Isolat KP 13 Dengan Metode Densitometri Dan Klt-Semprot. Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan, 4(1), 156–166. https://doi.org/10.36387/jiis.v4i1.263

Downloads

Published

2024-11-26

Issue

Vol. 9 No. 2 (2024): JIIS

Section

Article

License

Copyright (c) 2024 Jurnal Ilmiah Ibnu Sina

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM DARI EKSTRAK DAUN BANGUN-BANGUN (Coleus amboinicus L.) TERHADAP BAKTERI Staphylococcus aureus. (2024). JIIS (Jurnal Ilmiah Ibnu Sina): Ilmu Farmasi Dan Kesehatan, 9(2), 419-430. https://doi.org/10.36387/jiis.v9i2.2173

Similar Articles

31-40 of 48

You may also start an advanced similarity search for this article.